9Y58

Nef SF2 dimerization mutant bound to Hck SH3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033167
Spacegroup name	P 41 21 2
Unit cell lengths	84.325, 84.325, 83.162
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.710 - 2.250
R-factor	0.2399
Rwork	0.238
R-free	0.27250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.831
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.710	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.051	2.548
Rmeas	0.054	2.654
Rpim	0.015
Number of reflections	27154	34749
<I/σ(I)>	21.11
Completeness [%]	99.9	99.74
Redundancy	12.4	12.8
CC(1/2)	1.000	0.553

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M magnesium acetate, 0.1 M sodium acetate, pH 4.6, 25% PEG400