9WKU

Wild-type Menin complexed with JNJ-75276617

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.965459
Spacegroup name	P 1 21 1
Unit cell lengths	70.164, 78.357, 92.433
Unit cell angles	90.00, 101.62, 90.00

Refinement procedure

Resolution	42.240 - 1.900
R-factor	0.17555
Rwork	0.174
R-free	0.20415
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7uj4
RMSD bond length	0.010
RMSD bond angle	1.595
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.270	1.940
High resolution limit [Å]	1.900	1.900
Number of reflections	77039	4496
<I/σ(I)>	8
Completeness [%]	99.6
Redundancy	3.4
CC(1/2)	0.993	0.775

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	22%(w/v) PEG3350, 0.2M MAGNESIUM NITRATE, 0.1 M HEPES PH=7.8, 20%(v/v) ETHYLENE GLYCOL, PH7.8