Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-04-12 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.997, 57.544, 136.378 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.330 - 1.810 |
| R-factor | 0.2029 |
| Rwork | 0.200 |
| R-free | 0.25830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.190 | 1.910 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Number of reflections | 32651 | 4309 |
| <I/σ(I)> | 11.9 | |
| Completeness [%] | 98.7 | |
| Redundancy | 11.4 | |
| CC(1/2) | 0.999 | 0.561 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1M HEPES sodium pH7.5, 0.8M Sodium phosphate monobasic monohydrate, 0.8M Potassium phosphate monobasic |
