9WGM

Crystal structure of the OcuKAI2d2 protein from Orobanche cumana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	P 64 2 2
Unit cell lengths	149.799, 149.799, 56.854
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.080 - 2.190
Rwork	0.208
R-free	0.22660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.978
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0430 (REFMACAT 0.4.105))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.080	2.270
High resolution limit [Å]	2.190	2.190
Number of reflections	19763	1395
<I/σ(I)>	24.02	0.85
Completeness [%]	91.4	71.8
Redundancy	20	15
CC(1/2)	0.750	0.380

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2 M Magnesium chloride hexahydrate, 0.1 M Bis-Tris pH 6.5, 25% v/v PEG3350