9W69
Crystal structure of ASFV EP424R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.254, 57.939, 102.143 |
| Unit cell angles | 90.00, 112.16, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.790 |
| R-factor | 0.2051 |
| Rwork | 0.202 |
| R-free | 0.26200 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.124 |
| Data reduction software | HKL-2000 |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.940 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rmerge | 0.170 | |
| Rmeas | 0.211 | |
| Rpim | 0.122 | |
| Number of reflections | 21927 | 3185 |
| <I/σ(I)> | 3.6 | |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 2.8 | 2.8 |
| CC(1/2) | 0.955 | 0.731 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 16%(w/v) PEG 3350, 200 mM Ammonium citrate dibasic |
