9VX3
Crystal structure of the peptide-bound form of HisMab-1 Fv-clasp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.139, 69.512, 93.396 |
| Unit cell angles | 90.00, 93.78, 90.00 |
Refinement procedure
| Resolution | 39.570 - 2.390 |
| R-factor | 0.1976 |
| Rwork | 0.196 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.819 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.130 | 2.540 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Number of reflections | 94322 | 15415 |
| <I/σ(I)> | 11 | 1.21 |
| Completeness [%] | 99.0 | 97.3 |
| Redundancy | 3.47 | |
| CC(1/2) | 0.998 | 0.492 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 16% (w/v) polyethylene glycol 3350, 0.1M HEPES (pH 7.0), 0.2M MgCl2 |
