9VP7
Glucose-6-phosphate dehydrogenase from Leishmania donovani in complex with pseudo substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-24 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 191.840, 97.685, 70.449 |
| Unit cell angles | 90.00, 94.00, 90.00 |
Refinement procedure
| Resolution | 30.195 - 2.250 |
| Rwork | 0.220 |
| R-free | 0.28630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.320 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.195 | 2.310 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 61443 | 4530 |
| <I/σ(I)> | 6 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.7 | 7.2 |
| CC(1/2) | 0.960 | 0.917 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 4% Tacsimate, 12% PEG3350, 4-8% Glycerol |
