9VP6
Glucose-6-phosphate dehydrogenase from Leishmania donovani in complex with substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-14 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 193.396, 92.381, 70.628 |
| Unit cell angles | 90.00, 94.44, 90.00 |
Refinement procedure
| Resolution | 29.554 - 2.300 |
| Rwork | 0.228 |
| R-free | 0.31420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.284 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.554 | 9.760 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 54957 | 4522 |
| <I/σ(I)> | 6.3 | |
| Completeness [%] | 99.6 | |
| Redundancy | 7.4 | 8 |
| CC(1/2) | 0.990 | 0.937 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 4% Tacsimate, 12% PEG3350, 4-8% Glycerol |
