9VLF

Structural studies on the conformation changes induced by ligand binding in an Adenine phosphoribosyltransferase (FnAPRT) from Fusobacterium nucleatum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-14
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	73.048, 106.523, 47.807
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.020 - 1.610
R-factor	0.1942
Rwork	0.194
R-free	0.20550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.240
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.020	1.660
High resolution limit [Å]	1.610	1.610
Number of reflections	24571	2416
<I/σ(I)>	2.91
Completeness [%]	99.9
Redundancy	13.1
CC(1/2)	0.996	0.911

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	pH 8.2, 1.8M NaH2PO4/K2HPO4