9V4M
Crystal structure of the T.thermophilus transcription initiation complex bound to Ap4G
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-25 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.978950 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 185.055, 103.211, 296.509 |
| Unit cell angles | 90.00, 98.89, 90.00 |
Refinement procedure
| Resolution | 29.960 - 3.000 |
| R-factor | 0.2793 |
| Rwork | 0.279 |
| R-free | 0.28840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4q5s |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.909 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.126 | 1.240 |
| Rmeas | 0.143 | |
| Rpim | 0.065 | |
| Number of reflections | 105883 | 10426 |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 95.6 | 28.9 |
| Redundancy | 4.3 | 4.1 |
| CC(1/2) | 0.990 | 0.530 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 180 mM Kcl, 50 mM Mgcl2, 12.5% PEG4000, 100 mM Tris.Hcl pH 8.7 |
