9UVI

Crystal structure of beta 2 microglobulin in complex with antibody BBM.1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	126.15
Detector technology	CCD
Collection date	2024-12-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	59.990, 59.990, 187.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.310 - 2.500
R-factor	0.2018
Rwork	0.197
R-free	0.24570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.548
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.950	2.600
High resolution limit [Å]	2.500	2.500
Number of reflections	12659	1375
<I/σ(I)>	15.2
Completeness [%]	100.0	100
Redundancy	19.9
CC(1/2)	0.998	0.985

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.2 M Sodium acetate trihydrate 0.1 M Bis-Tris propane 8.5 20 % w/v PEG 3350