9UTM

Crystal structure of an anti-CRISPR protein AcrIIA11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 62
Unit cell lengths	105.733, 105.733, 89.757
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.300 - 3.200
R-factor	0.2448
Rwork	0.243
R-free	0.28580
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.783
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.300	3.300
High resolution limit [Å]	3.200	3.200
Number of reflections	9545	925
<I/σ(I)>	17.7
Completeness [%]	99.5
Redundancy	40.6
CC(1/2)	0.960	0.718

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 3350, Tris, magnesium chloride