9UHD

Structure of FAM92A BAR domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97923
Spacegroup name	P 31 2 1
Unit cell lengths	132.270, 132.270, 67.760
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	58.320 - 2.210
R-factor	0.1894
Rwork	0.188
R-free	0.21490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.144
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.320	2.270
High resolution limit [Å]	2.210	2.210
Rmerge	0.189	2.499
Rmeas	0.194	2.564
Rpim	0.044	0.571
Total number of observations	677791	50858
Number of reflections	34469	2526
<I/σ(I)>	17.5	1.6
Completeness [%]	100.0
Redundancy	19.7	20.1
CC(1/2)	0.999	0.713

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	15% w/v polyethylene glycol 3350, 10 mM magnesium chloride hexahydrate and 5 mM nickel (II) chloride hexahydrate, pH 7.0