9UHD
Structure of FAM92A BAR domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-10-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97923 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 132.270, 132.270, 67.760 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 58.320 - 2.210 |
R-factor | 0.1894 |
Rwork | 0.188 |
R-free | 0.21490 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.144 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.320 | 2.270 |
High resolution limit [Å] | 2.210 | 2.210 |
Rmerge | 0.189 | 2.499 |
Rmeas | 0.194 | 2.564 |
Rpim | 0.044 | 0.571 |
Total number of observations | 677791 | 50858 |
Number of reflections | 34469 | 2526 |
<I/σ(I)> | 17.5 | 1.6 |
Completeness [%] | 100.0 | |
Redundancy | 19.7 | 20.1 |
CC(1/2) | 0.999 | 0.713 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 15% w/v polyethylene glycol 3350, 10 mM magnesium chloride hexahydrate and 5 mM nickel (II) chloride hexahydrate, pH 7.0 |