9UC5

Mini-protein binder of N-terminal domain of nucleocapsid protein of SARS-CoV2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	100
Detector technology	CCD
Collection date	2025-01-08
Detector	RAYONIX MX-225
Wavelength(s)	0.97777
Spacegroup name	P 21 21 21
Unit cell lengths	39.780, 42.460, 95.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.570 - 1.800
R-factor	0.2135
Rwork	0.212
R-free	0.23290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.655
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (v21.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.570	1.840
High resolution limit [Å]	1.800	1.800
Number of reflections	15592	889
<I/σ(I)>	15.4
Completeness [%]	99.9	100
Redundancy	6.8
CC(1/2)	0.996	0.931

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	294	0.15M Potassium bromide, 30%PEG MME 2000