9UBI
Crystal structure of FERONIA kinase and Ferovicin inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-05-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.794, 83.114, 61.993 |
| Unit cell angles | 90.00, 93.38, 90.00 |
Refinement procedure
| Resolution | 49.640 - 1.900 |
| R-factor | 0.1722 |
| Rwork | 0.171 |
| R-free | 0.20260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.225 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.640 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 95692 | 2565 |
| <I/σ(I)> | 5.25 | |
| Completeness [%] | 99.4 | 98.54 |
| Redundancy | 5.4 | |
| CC(1/2) | 0.990 | 0.890 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291.15 | 0.3 M ammonium sulfate, 0.1 M Bis-Tris (pH 7.0), and 20% PEG mme 3350 |
