9TUY

PfRIPR EGF6 and 7 bound to monoclonal antibody MAD8-739

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.6199
Spacegroup name	P 31
Unit cell lengths	89.637, 89.637, 141.336
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.020 - 2.800
R-factor	0.2292
Rwork	0.228
R-free	0.25480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.950
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.4 (10-JUL-2024))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.720	2.950
High resolution limit [Å]	2.800	2.800
Number of reflections	31298	4549
<I/σ(I)>	5.6
Completeness [%]	100.0
Redundancy	10.7
CC(1/2)	0.993	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M sodium cacodylate pH 6.0, 15% w/v PEG 4000