9TOS

Drop on fixed target room temperature serial crystal structure of hen egg white lysozyme, drop on fixed target at DLS, 1.9 s checkerboard control (N-acetyl glucosamine).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	293
Detector technology	PIXEL
Collection date	2025-06-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9998
Spacegroup name	P 43 21 2
Unit cell lengths	78.961, 78.961, 38.262
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.830 - 1.670
R-factor	0.181
Rwork	0.179
R-free	0.21830
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.005
RMSD bond angle	0.736
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHENIX
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.840	1.699
High resolution limit [Å]	1.670	1.670
Number of reflections	14565	704
<I/σ(I)>	2.342	0.795
Completeness [%]	100.0	99.86
Redundancy	48.17	22.92
CC(1/2)	0.984	0.342

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		295	1 M citric acid, 20% (w/v) NaCl, 5% (w/v) PEG 6000, pH 3.0.