9TJV
Ternary complex of E. coli leucyl-tRNA synthetase mutant W177A, tRNA(leu) and the leucyl adenylate analog LeuAMS in the aminoacyl transfer state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-07 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.87313 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 158.009, 68.312, 226.276 |
| Unit cell angles | 90.00, 104.44, 90.00 |
Refinement procedure
| Resolution | 219.130 - 2.277 |
| R-factor | 0.1953 |
| Rwork | 0.194 |
| R-free | 0.22240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.810 |
| Data reduction software | autoPROC (20240123) |
| Data scaling software | STARANISO |
| Phasing software | PHASER (2.8.3) |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 219.132 | 2.490 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.127 | 0.773 |
| Rmeas | 0.152 | 0.915 |
| Rpim | 0.083 | 0.486 |
| Number of reflections | 84189 | 4209 |
| <I/σ(I)> | 5.2 | 1.5 |
| Completeness [%] | 92.0 | 43.9 |
| Redundancy | 3.3 | 3.4 |
| CC(1/2) | 0.992 | 0.591 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1M BisTris pH5.5, 0.2M Ammonium Acetate, 23-26% PEG3350 |
