9TJU
Ternary complex of E. coli leucyl-tRNA synthetase bound to tRNA(leu) and Leucinol in the editing state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.96546 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.694, 119.545, 141.639 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.410 - 1.782 |
| R-factor | 0.2049 |
| Rwork | 0.203 |
| R-free | 0.23470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.850 |
| Data reduction software | autoPROC (20240123) |
| Data scaling software | STARANISO |
| Phasing software | PHASER (2.8.3) |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.820 | 1.917 |
| High resolution limit [Å] | 1.782 | 1.782 |
| Rmerge | 0.093 | 1.548 |
| Rmeas | 0.097 | 1.615 |
| Rpim | 0.027 | 0.455 |
| Number of reflections | 101703 | 5085 |
| <I/σ(I)> | 15.4 | 1.5 |
| Completeness [%] | 96.3 | 66.7 |
| Redundancy | 13.2 | 12.4 |
| CC(1/2) | 0.997 | 0.643 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.1M Sodium Acetate pH5.0, 0.2M Ammonium Chloride, 20-24% PEG 6000 |
