9TF5
Structure of human wild-type signal regulatory protein alpha V2 variant (SIRPaV2) with zinc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 57.263, 57.263, 199.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.360 - 1.630 |
| R-factor | 0.1895 |
| Rwork | 0.188 |
| R-free | 0.21630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.997 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.360 | 1.660 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.117 | 0.730 |
| Rmeas | 0.841 | |
| Rpim | 0.032 | 0.405 |
| Number of reflections | 76899 | 1613 |
| <I/σ(I)> | 8 | 2.9 |
| Completeness [%] | 98.3 | 78.4 |
| Redundancy | 8.9 | 3.5 |
| CC(1/2) | 0.554 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.01 M zinc sulfate heptahydrate, 0.1M MES monohydrate pH 6.5, and 25% (v/v) PEGMME 550 |
