9TB5

Crystal structure of the MpPYL1-ABA-HAB1 ternary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97926
Spacegroup name	P 21 21 21
Unit cell lengths	70.060, 71.997, 103.546
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.180 - 1.980
R-factor	0.2297
Rwork	0.226
R-free	0.29140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.862
Data reduction software	XDS
Data scaling software	XDS
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.180	2.030
High resolution limit [Å]	1.980	1.980
Number of reflections	37271	2502
<I/σ(I)>	0.654
Completeness [%]	99.7
Redundancy	9.8
CC(1/2)	0.923	0.438

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.9	291	0.2 M magnesium formate, pH 5.9, 20% (w/v) PEG 3350