9T9W

Crystal structure of beta-TrCP bound by diphosphorylated I-kappa-B-alpha degron peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95373
Spacegroup name	P 21 21 21
Unit cell lengths	44.602, 46.822, 150.381
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.130 - 1.162
R-factor	0.1911
Rwork	0.190
R-free	0.21260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.280
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	BUSTER (2.11.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.705	1.277
High resolution limit [Å]	1.162	1.162
Number of reflections	86137	4307
<I/σ(I)>	6.9
Completeness [%]	94.3
Redundancy	6.2
CC(1/2)	0.992	0.412

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	18% PEG8K