9T2D
Jumonji domain-containing protein 2B with crystallization epitope mutations L916G:R917A:A918D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92819 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 88.850, 88.850, 143.860 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.580 - 1.800 |
| R-factor | 0.2377 |
| Rwork | 0.237 |
| R-free | 0.25530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.910 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.960 | 1.780 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.047 | 1.593 |
| Number of reflections | 20481 | 580 |
| <I/σ(I)> | 17.2 | |
| Completeness [%] | 100.0 | |
| Redundancy | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277.15 | 2M ammonium sulfate 2% PEG400 0.1M HEPES pH 7.5 |
