9T00
Crystal structure of prethrombin-2 with a peptide corresponding to the C-terminus of the heavy chain of factor Va
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-10-09 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.7838 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.416, 51.567, 65.992 |
| Unit cell angles | 82.90, 85.33, 65.90 |
Refinement procedure
| Resolution | 38.760 - 2.800 |
| Rwork | 0.259 |
| R-free | 0.30450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.785 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0431) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.760 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.244 | 1.032 |
| Number of reflections | 15006 | 2190 |
| <I/σ(I)> | 3 | 0.7 |
| Completeness [%] | 99.2 | 98.9 |
| Redundancy | 3.7 | 3.4 |
| CC(1/2) | 0.950 | 0.432 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.2 M NaCl (Salt) 0.1 M TRIS 8.5 pH (Buffer) 25 %w/v PEG 3350 (Precipitant) |
