9SWJ
Human ADP-forming succinyl-CoA ligase complex SUCLG1-SUCLA2 bound to coenzyme A
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 QUANTUM (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.7 |
| Number of Particle | 320000 |
| Software | cryoSPARC,PHENIX,EPU,UCSF ChimeraX |
| Symmetry | D2 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
