9SPN

p53 cancer mutant V157F in complex with DARPin C10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	38.200, 92.896, 53.735
Unit cell angles	90.00, 108.17, 90.00

Refinement procedure

Resolution	46.450 - 1.930
R-factor	0.192869798316
Rwork	0.191
R-free	0.22307
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.007
RMSD bond angle	0.833
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.450	1.980
High resolution limit [Å]	1.930	1.930
Rmerge	0.074	0.863
Number of reflections	26770	1785
<I/σ(I)>	12.2	2
Completeness [%]	99.9	100
Redundancy	5.1	5.2
CC(1/2)	0.998	0.745

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Protein solution: 0.6 mg/ml DARPin-p53 complex in 25 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP. Reservoir solution: 20% PEG3350, 10% ethylene glycol, 0.2 M sodium/potassium phosphate. Drop volume ratio 2:1.