9SNP

VHL/Elongin B/Elongin C complex with compound 60

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-23
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9121
Spacegroup name	P 41 2 2
Unit cell lengths	94.019, 94.019, 364.477
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.120 - 2.700
R-factor	0.2282
Rwork	0.227
R-free	0.25890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.526
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.120	2.790
High resolution limit [Å]	2.700	2.700
Rmerge	0.089	0.746
Rmeas	0.096	0.804
Rpim	0.036	0.299
Number of reflections	46272	4402
<I/σ(I)>	18.6	3.5
Completeness [%]	100.0	100
Redundancy	13.1
CC(1/2)	0.999	0.936

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	100 mM Sodium Citrate pH 6.0, 200 mM Magnesium Acetate, 50 mM DTT, 14% PEG 8000