9SMO
Crystal Structure of human exonuclease 1 (Exo1) with DNA and compound ART5537
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-08 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 69.705, 69.705, 181.759 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.080 - 2.199 |
| R-factor | 0.2404 |
| Rwork | 0.239 |
| R-free | 0.27060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.910 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC ((Version 1.1.7)) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8 (11-DEC-2020)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.083 | 2.503 |
| High resolution limit [Å] | 2.199 | 2.199 |
| Rmerge | 0.166 | 2.351 |
| Number of reflections | 13546 | 677 |
| <I/σ(I)> | 14 | 1.6 |
| Completeness [%] | 57.3 | 9.1 |
| Redundancy | 24.9 | 24.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20% PEG 3350, 0.2M potassium sulfate |
