9SMD
Ribosome-dome complex including the Sec-translocon (SecYEG-SecA-SecDF) from antibiotics treated Mycoplasma pneumoniae cells (Combined)
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K2 SUMMIT (4k x 4k),GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SUBTOMOGRAM AVERAGING |
| Resolution (Å) | 9.4 |
| Software | PyTom,RELION,Warp,SerialEM |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING ONLY |
