9SI8
Structure of trans-basal conformer of human CBS trapped in PLP-aminoacrylate intermediate state (CBS PLP-AA)- by Helical processing.
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 2.47 |
| Software | cryoSPARC,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
