9SG0

CJM112 Fv in complex with human IL-17A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99984
Spacegroup name	C 2 2 21
Unit cell lengths	55.720, 87.030, 156.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	78.130 - 2.650
R-factor	0.198
Rwork	0.195
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.270
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	78.130	78.130	2.720
High resolution limit [Å]	2.650	11.850	2.650
Rmerge	0.073	0.025	3.726
Rmeas	0.079	0.028	4.037
Number of reflections	11404	152	844
<I/σ(I)>	13.2	45.76	0.5
Completeness [%]	99.9	98.7	100
Redundancy	6.5	5.14	6.77
CC(1/2)	0.999	0.999	0.330

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	292	0.1M MES pH 6.5, 0.2M ammonium sulfate, 15% PEG 5,000 MME