9SFX

Secukinumab Fv in complex with human IL-17A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-06-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99996
Spacegroup name	P 61 2 2
Unit cell lengths	186.350, 186.350, 107.970
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.680 - 2.600
R-factor	0.1783
Rwork	0.176
R-free	0.21270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.200
Data reduction software	XDS
Data scaling software	XSCALE (July 4, 2012)
Phasing software	PHASER
Refinement software	BUSTER (2.11.4)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.680	27.680	2.670
High resolution limit [Å]	2.600	11.630	2.600
Rmerge	0.131	0.030	2.888
Rmeas	0.134	0.031	2.958
Number of reflections	34093	467	2458
<I/σ(I)>	23.46	77.81	1.73
Completeness [%]	99.0	98.7	98.6
Redundancy	20.1	15.94	21
CC(1/2)	0.999	1.000	0.702

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	292	0.1M sodium acetate pH 4.6, 1.8M ammonium citrate dibasic