9SDF
Structure at 1.9 A resolution of Thermus thermophilus tyrosyl-tRNA synthetase bound to wild-type modified tRNA(Tyr), tyrosine and AMP
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-29 |
| Detector | MAR CCD 300 mm |
| Wavelength(s) | 0.97625 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.067, 67.378, 113.817 |
| Unit cell angles | 90.00, 112.25, 90.00 |
Refinement procedure
| Resolution | 16.740 - 1.900 |
| R-factor | 0.189 |
| Rwork | 0.188 |
| R-free | 0.21390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.895 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.440 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.099 | 0.518 |
| Number of reflections | 127578 | 5005 |
| <I/σ(I)> | 8.18 | |
| Completeness [%] | 99.1 | 99.1 |
| Redundancy | 2.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | do not know Cryo: 22% PEG 400 |
