9SBB

Monoclonal Antibodies from COVID-19 Convalescent Patients Target Cryptic Epitopes for Universal SARS-CoV-2 Neutralization

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-03-04
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.8731
Spacegroup name	C 1 2 1
Unit cell lengths	111.797, 87.975, 95.763
Unit cell angles	90.00, 108.25, 90.00

Refinement procedure

Resolution	60.420 - 2.120
R-factor	0.1988
Rwork	0.197
R-free	0.23020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.470
Data reduction software	TRUNCATE
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (v1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	60.420	2.630	2.160
High resolution limit [Å]	2.120	2.530	2.120
Rmerge	0.130	0.434	1.625
Rmeas	0.154	0.595
Rpim	0.082
Number of reflections	49442	5798	2730
<I/σ(I)>	6.09		0.84
Completeness [%]	98.6
Redundancy	3.4
CC(1/2)	0.993	0.742	0.372

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	0.2M Lithium Citrate Tribasic Tetrahydrate, 20% PEG 3350