9SAT

Monoclonal Antibodies from COVID-19 Convalescent Patients Target Cryptic Epitopes for Universal SARS-CoV-2 Neutralization

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-04
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.8371
Spacegroup name	I 21 21 21
Unit cell lengths	83.264, 102.996, 225.499
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	93.690 - 2.560
R-factor	0.2331
Rwork	0.231
R-free	0.27530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.455
Data reduction software	TRUNCATE
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (v1.21)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.690	2.650
High resolution limit [Å]	2.560	2.560
Rmerge	0.067	0.232
Rmeas	0.095	0.328
Rpim	0.067	0.232
Number of reflections	28734	2658
<I/σ(I)>	9.39	3.65
Completeness [%]	90.7	85.56
Redundancy	1.9
CC(1/2)	0.990	0.838

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	289.15	0.2M Zinc Acetate Dehydrate, 0.1M Sodium Cacodylate Trihydrate, 15% PEG 8000