9RWI
crystal structure of a human leukocyte antigen-A (HLA-G) blocking antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-05-19 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.976246 |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 165.495, 165.495, 105.819 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 74.010 - 3.450 |
| R-factor | 0.2571 |
| Rwork | 0.255 |
| R-free | 0.29280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.567 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.010 | 3.780 |
| High resolution limit [Å] | 3.450 | 3.450 |
| Rmeas | 0.150 | |
| Rpim | 0.029 | 0.380 |
| Number of reflections | 18646 | 4094 |
| <I/σ(I)> | 2.1 | |
| Completeness [%] | 93.4 | 87.5 |
| Redundancy | 26.1 | 25.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2M Ammonium sulfate and 0.1 M Tris at pH 8.0 |
