9RTX
Mammalian AP3 complex on tubular membranes (ARF1 centered Beta3-ARF1 dimer-Beta3 interface)
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Energy Filter | TFS Selectris X |
| Detector | TFS FALCON 4i (4k x 4k) |
3D Reconstruction
| Method | SUBTOMOGRAM AVERAGING |
| Resolution (Å) | 8.5 |
| Software | UCSF Chimera,UCSF ChimeraX,RELION,subTOM,Warp,M |
| Symmetry | C2 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
