9RM2
BKPyV VP1 IN COMPLEX WITH 319C07-FAB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-07 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 109.349, 172.078, 113.144 |
| Unit cell angles | 90.00, 97.64, 90.00 |
Refinement procedure
| Resolution | 91.870 - 1.960 |
| R-factor | 0.18892 |
| Rwork | 0.187 |
| R-free | 0.21819 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.361 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC ((Version 1.1.7)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.870 | 1.991 |
| High resolution limit [Å] | 1.957 | 1.957 |
| Rmerge | 0.094 | 1.220 |
| Number of reflections | 295460 | 295460 |
| <I/σ(I)> | 8.5 | 1.3 |
| Completeness [%] | 99.4 | 98.7 |
| Redundancy | 4.4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M MIB buffer pH 4.75, 21% (w/v) PEG 1500 |
