9RFA
Apo structure of glxR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-08-01 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.442, 121.465, 132.835 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.730 - 2.069 |
| R-factor | 0.2712 |
| Rwork | 0.270 |
| R-free | 0.31500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4 (10-JUL-2024)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.730 | 2.100 |
| High resolution limit [Å] | 2.069 | 2.070 |
| Rmerge | 0.218 | 4.611 |
| Rmeas | 0.227 | 4.793 |
| Rpim | 0.061 | 1.301 |
| Total number of observations | 52372 | |
| Number of reflections | 80069 | 3916 |
| <I/σ(I)> | 6.7 | 0.3 |
| Completeness [%] | 100.0 | |
| Redundancy | 13.6 | 13.4 |
| CC(1/2) | 0.992 | 0.402 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 18 %v/v PEGSH 0.2 M (NH4)2SO4, 0.1 M ADA 6.5 pH |
