9RD6

Crystal structure of the 4CHRD domain of human chordin with Anderson-Evans polyoxotungstate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	99
Detector technology	PIXEL
Collection date	2025-01-25
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9537
Spacegroup name	P 43 2 2
Unit cell lengths	91.302, 91.302, 323.657
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	79.520 - 3.280
R-factor	0.2114
Rwork	0.210
R-free	0.24690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.619
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.910	3.540
High resolution limit [Å]	3.280	3.280
Rmeas	0.447
Rpim	0.064	1.367
Number of reflections	44114	8840
<I/σ(I)>	9.3	1.1
Completeness [%]	100.0	100
Redundancy	48.6	38.9
CC(1/2)	1.000	0.522

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277.15	0.1M Morpheus buffer system 3 (Tris/Bicine) pH 8.0, 0.1M Morpheus amino acids (DL-Glutamic acid monohydrate, DL-Alanine, Glycine, DL-Serine and DL-Lysine monohydrochloride). Morpheus condition H11 with 1mM TEW and 0.05 mg/mL Trypsin
2	VAPOR DIFFUSION, SITTING DROP	8.5	277.15	0.1M Morpheus buffer system 3 (Tris/Bicine) pH 8.0, 0.1M Morpheus amino acids (DL-Glutamic acid monohydrate, DL-Alanine, Glycine, DL-Serine and DL-Lysine monohydrochloride). Morpheus condition H11 with 1mM TEW and 0.05 mg/mL Trypsin