9RAI
Structure of the S.aureus MecA/ClpC/ClpP degradation system
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3 |
| Number of Particle | 2851121 |
| Software | Topaz,Servalcat,cryoSPARC,EPU,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
