9R6E

Structural characterization of a N-Acetyl-D-glucosamine-binding site in human RNase2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	41.829, 52.621, 56.506
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.510 - 1.020
R-factor	0.1268
Rwork	0.126
R-free	0.13650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.058
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.510	1.056
High resolution limit [Å]	1.020	1.020
Number of reflections	49535	751
<I/σ(I)>	1.04
Completeness [%]	77.1
Redundancy	4.3
CC(1/2)	0.998	0.385

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.22M Sodium formate 0.1M Bis-Tris propane 12% PEG 3350 PH=8.7