9R2E
Structure of ARGX-121 Fab fragment in complex with the Fc fragment of IgA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-01-20 |
| Detector | DECTRIS EIGER2 X CdTe 16M |
| Wavelength(s) | 0.885603 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.291, 232.312, 105.519 |
| Unit cell angles | 90.00, 90.35, 90.00 |
Refinement procedure
| Resolution | 116.160 - 2.540 |
| R-factor | 0.21306 |
| Rwork | 0.211 |
| R-free | 0.25182 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC (1.1.7) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 116.160 | 2.583 |
| High resolution limit [Å] | 2.539 | 2.539 |
| Rmerge | 0.129 | 1.592 |
| Number of reflections | 59436 | 3039 |
| <I/σ(I)> | 4.1 | 1.3 |
| Completeness [%] | 97.2 | 99.7 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 16% (w/v) PEG8000, 20% (v/v) Glycerol, 0.16 M MgAcetate, 0.08 M Na Cacodylate pH 6.50 |
