9QY8
Crystal structure of apoform human USP18
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-04 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.868, 71.775, 73.063 |
| Unit cell angles | 79.32, 76.28, 89.88 |
Refinement procedure
| Resolution | 39.593 - 1.919 |
| Rwork | 0.205 |
| R-free | 0.26010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.724 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.593 | 1.950 |
| High resolution limit [Å] | 1.919 | 1.920 |
| Rmerge | 0.071 | 2.455 |
| Number of reflections | 69489 | 3316 |
| <I/σ(I)> | 17.9 | |
| Completeness [%] | 97.9 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.996 | 0.342 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.2 M sodium citrate tribasic dihydrate, 0.1 M Bis-Tris propane, pH 7.5, 20 % (w/v) PEG3350 |
