Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-25 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9201 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.220, 66.877, 156.909 |
| Unit cell angles | 90.00, 99.29, 90.00 |
Refinement procedure
| Resolution | 34.940 - 1.490 |
| Rwork | 0.163 |
| R-free | 0.20650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.316 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430 (refmacat 0.4.100)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.940 | 1.530 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.071 | 0.768 |
| Rmeas | 0.084 | 0.913 |
| Rpim | 0.044 | 0.488 |
| Number of reflections | 260713 | 13037 |
| <I/σ(I)> | 9.9 | 1.5 |
| Completeness [%] | 95.0 | 68.1 |
| Redundancy | 3.5 | 3.4 |
| CC(1/2) | 1.000 | 0.540 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 25-30% PEG 3350, 200 mM KSCN and 100 mM bis-tris propane pH 7.5 |
