9Q4D

A DARPin fused to the double trigger 1TEL variant crystallization chaperone via a direct helical fusion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-06-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979460
Spacegroup name	P 65
Unit cell lengths	83.021, 83.021, 56.840
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.950 - 1.302
R-factor	0.158
Rwork	0.156
R-free	0.18540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.945
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PDB-REDO

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.950	1.348
High resolution limit [Å]	1.302	1.302
Rmerge	0.113	3.614
Rmeas	0.116	3.750
Rpim	0.025	0.974
Number of reflections	51538	3440
<I/σ(I)>	12.66	0.62
Completeness [%]	94.0	63.61
Redundancy	19.9	13.6
CC(1/2)	0.999	0.346

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	0.07M Citric acid, 0.05M BIS-TRIS propane, 16% w/v Polyethylene Glycol 3,350