9Q3N

Crystal structure of human CDK5-cyclinB1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979460
Spacegroup name	P 32 2 1
Unit cell lengths	121.583, 121.583, 115.988
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.980 - 2.100
R-factor	0.1725
Rwork	0.171
R-free	0.22290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.610
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.21_5207: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.980	2.160
High resolution limit [Å]	2.100	2.100
Number of reflections	58032	4423
<I/σ(I)>	13.1
Completeness [%]	99.8	99.2
Redundancy	10.2
CC(1/2)	0.999	0.281

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	10% PEG 3350 200 mM Imidazole/Malate pH 7.8 5% Mg Formate (saturated)