9Q1O
Crystal structure of human TEAD2-Yap binding domain covalently bound to an allosteric regulator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2020-09-24 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 1.00003 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.898, 61.666, 79.766 |
| Unit cell angles | 90.00, 117.52, 90.00 |
Refinement procedure
| Resolution | 53.560 - 2.260 |
| R-factor | 0.2115 |
| Rwork | 0.209 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6e5g |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.511 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.560 | 2.570 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmeas | 0.146 | 0.961 |
| Rpim | 0.076 | 0.508 |
| Number of reflections | 12980 | 650 |
| <I/σ(I)> | 8.8 | 1.5 |
| Completeness [%] | 88.1 | 66.1 |
| Redundancy | 3.7 | 3.4 |
| CC(1/2) | 0.994 | 0.713 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Sodium Formate 1.8M - 2.4M HEPES pH 7.2 - 7.4 |
