Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97741 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 86.630, 109.420, 141.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.930 - 1.930 |
| R-factor | 0.2485 |
| Rwork | 0.247 |
| R-free | 0.28260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.169 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.930 | 2.000 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.105 | 1.082 |
| Rmeas | 0.114 | 1.196 |
| Rpim | 0.044 | 0.466 |
| Number of reflections | 50246 | 4832 |
| <I/σ(I)> | 11.2 | 1.78 |
| Completeness [%] | 99.6 | 97.52 |
| Redundancy | 6.6 | 6.4 |
| CC(1/2) | 0.999 | 0.458 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293.15 | 100 mM sodium acetate, 2.0M ammonium sulfate |
