9PRU

Complex of the 3G8 Fab bound to Fc gamma receptor 3a / CD16a F158 allotype

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 19-ID
Synchrotron site	NSLS-II
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-01-05
Detector	DECTRIS EIGER2 XE 9M
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	130.062, 130.594, 170.511
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.000 - 1.900
R-factor	0.23
Rwork	0.230
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.21.2_5419)
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.046	0.501
Number of reflections	230340	1
<I/σ(I)>	19.8
Completeness [%]	100.0
Redundancy	6.1
CC(1/2)	0.940	0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.82	291	0.2 M KCl, 0.1 M Bis-Tris, 20% PEG3350, 10% glycerol